Open AccessA method for the study of fungal growth inhibition by plant proteins
Author(s) -
Ludwig Alexander,
Boller Thomas
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04175.x
Subject(s) - bioassay , chitinase , fungal growth , spore germination , germination , spore , growth inhibition , enzyme , glucanase , biology , plant growth , biochemistry , chromatography , chemistry , microbiology and biotechnology , botany , in vitro , genetics
A bioassay is described for the study of inhibitory activity of plant proteins on fungal growth. Fungal spores were germinated in liquid growth medium and pipetted into wells of a microtitre plate. Fungal growth was followed spectrophotometrically. The bioassay was tested using crude protein extracts from plant tissues known to have high activities of chitinase and β‐1,3‐glucanase, and with purified enzymes. Crude protein preparations and combinations of the purified enzymes produced a temporary reduction of growth but no permanent growth inhibition.