Open AccessAn automated quantitative assay for fungal growth inhibition
Author(s) -
Broekaert Willem F.,
Terras Frankt R.G.,
Cammue Bruno P.A.,
Vanderleyden Jos
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04174.x
Subject(s) - absorbance , relative standard deviation , chromatography , spore , standard curve , volume (thermodynamics) , chemistry , fungal growth , analytical chemistry (journal) , biology , microbiology and biotechnology , detection limit , physics , quantum mechanics
A simple technique which enables the monitoring of fungal growth with the aid of a microplate reader is described. In the absorbance range of 0 to 0.6 units, a straight‐line relationship exists between absorbance at 595 nm and dry weight of microplate cultures, indicating that culture absorbance is an accurate indicator of fungal biomass. The relative standard deviation of the absorbance measurements was low (typically between 2 and 6%) when spores were used for inoculum. With inoculi consisting of mycelial fragments, slightly higher standard deviations (ca. 10%) were found. The microplate reader technique is particularly suited for determination of growth inhibition curves, since it is extremely fast, reliable, and requires as little as 75 μl of total culture volume.