Open AccessFluid‐phase endocytosis in yeasts other than Saccharomyces cerevisiae
Author(s) -
Fernandez Nieves,
Puente Pilar,
Leal Fernando
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04166.x
Subject(s) - yarrowia , endocytosis , internalization , schizosaccharomyces pombe , saccharomyces cerevisiae , candida albicans , yeast , debaryomyces hansenii , vacuole , extracellular , biology , kluyveromyces , dextran , microbiology and biotechnology , biochemistry , kluyveromyces lactis , endocytic cycle , candida parapsilosis , receptor , cytoplasm
A FITC‐dextran internalization assay with Saccharomyces cerevisiae as positive control was used to determine whether fluid‐phase endocytosis is a general characteristic of yeasts. Schizosaccharomyces pombe, Pichia polymorpha, Kluyveromyces phaseolosporus, Yarrowia lipolytica and Candida albicans were clearly positive, whereas results obtained with Dev̇aryomyces marama were inconclusive. In all cases internalized FITC‐dextran was found to be localized in the vacuoles and the process was always time‐ and temperature‐dependent. Lower eucaryotes, particularly yeasts, appear to have the ability to incorporate substances from the extracellular medium through fluid‐phase endocytosis.