Open AccessNMR studies of a bacterial cell culture medium (LB Broth): cyclic nucleosides in yeast extracts
Author(s) -
Rayner Michael H.,
Sadler Peter J.,
Scawen Michael D.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04152.x
Subject(s) - yeast , autolysis (biology) , rnase p , yeast extract , fast protein liquid chromatography , nucleotide , nuclear magnetic resonance spectroscopy , biochemistry , saccharomyces cerevisiae , chemistry , gel electrophoresis , rna , stereochemistry , enzyme , fermentation , gene
The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by 1 H, 31 P‐NMR spectroscopy, FPLC and gel electrophoresis. An enexpected finding was the high level of 2′,3′‐cyclic nucleotides, detected by characteristic 31 P‐NMR resonances in the region 20–21 ppm, originating from the yeast component. 31 P‐NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA.