Open AccessTransfer and expression of the tetracyclin resistance transposon Tn 925 in Acetobacterium woodii
Author(s) -
Strätz Michael,
Gottschalk Gerhard,
Dürre Peter
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04144.x
Subject(s) - transposable element , biology , resistance (ecology) , genetics , gene , ecology , genome
The Enterococcus faecalis conjugative plasmid pCF10 was used to introduce Tn 925 into Acetobacterium woodii by filter mating. Tetracycline resistance was transferred at frequencies of about 10 −6 per donor, but no plasmid DNA was found in the transconjugants. DNA hybridization analyses of Hin dIII‐digested chromosomal DNA demonstrated the insertion of Tn 925 at a variety of locations, whereas wild type DNA showed no hybridization at all. The transconjugants were used as donor in mating experiments with tetracycline‐sensitive Bacillus subtilis . Transfer of tetracycline resistance was observed at frequencies of 10 −8 per recipient.