Open AccessAnalysis of the 2‐keto‐3‐deoxyoctonate (KDO) region of lipopolysaccharides isolated from non‐01 Vibrio cholerae 05R
Author(s) -
Kondo Seiichi,
Haishima Yuji,
Hisatsune Kazuhito
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04140.x
Subject(s) - vibrio cholerae , microbiology and biotechnology , vibrio , vibrionaceae , cholera , biology , bacteria , genetics
Phosphorylated 2‐keto‐deoxyoctonate (KDO) has been detected in the stronga‐acid hydrolysates of lipopolysaccharides (LPS) of family Vibroonaceae including Vibrio cholerae . Structural analysis of LPS isolated from a rough mutant of non‐01 V. cholerae 05 by dephosphorylation, periodate oxidation and methylation analysis revealed that the inner core region of the LPS molecule contains only one mole of KDO in contrast to enteric Gram‐negative bacterial LPS, and that the phosphage group on the KDO molecule resides in the C4 position, while the site of binding of KDO to heptose, a constituent of the distal part of the inner core region, is the C5 position as in the enteric bacterial LPS.