Open AccessEnhanced cytochrome formation and stimulate methanogenesis rate by the increased ferrous concentrations in Methanosarcina barkeri culture
Author(s) -
Lin Dong,
Kakizono Toshihide,
Nishio Naomichi,
Nagai Shiro
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04128.x
Subject(s) - methanosarcina barkeri , ferrous , cytochrome , methanogenesis , chemistry , biochemistry , methanosarcina , excretion , methanol , chromatography , methane , enzyme , organic chemistry
The concentration of Fe 2+ in the methanol minimum medium was found to be a stimulating factor on the formation of cytochromes, the excretion of hemes and the rate of methane formation by Methanosarcino barkeri cells. Overproduced hemes due to the high Fe 2+ concentration were released into the medium as free forms. When Fe 2+ concentration was employed more than 30 μM for the medium, the contents of cytochrome b and c in the cells seemed to be saturated at 40 nmol/g cell. Under the same condition, not only membrane‐bound but also soluble cytochromes were detected in the ultracentrifuged fractions from the cells grown on 35 μM of Fe 2+