Open AccessAn outer membrane protein characteristic of mucoid strains of Pseudomonas aeruginosa
Author(s) -
Grabert Elmar,
Wingeder Jost,
Winkler Ulrich K.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04127.x
Subject(s) - bacterial outer membrane , pseudomonas aeruginosa , polyacrylamide gel electrophoresis , electrophoresis , gel electrophoresis , pseudomonadaceae , lipopolysaccharide , bacteria , pseudomonadales , membrane protein , biology , solubilization , biochemistry , membrane , microbiology and biotechnology , chemistry , escherichia coli , enzyme , gene , genetics , endocrinology
SDS‐polyacrylamide gel electrophoresis of outer membrane (OM) proteins of different mucoid strains of P. aeruginosa revealed a portion of about 54 kDa that was absent in nonmucoid strains. This 54 kDa protein was expressed under iron‐restricted and ironsufficient growth conditions. Electrophoretic mobility of the 54 kDa protein was modified by the solubilization temperature as well as by the addition of lipopolysaccharide and alginate prior to electrophoresis. Treatment of OMs with ocytlglucoside/KCl or SDS completely extracted the 54 kDa protein at low temperatures. The possible role of this protein in biosynthesis and/or excretion of bacterial alginate is discussed.