Open AccessEffect of iron limitation on protein composition and ultrastructure of Haemophilus ducreyi
Author(s) -
Abeck D.,
Johnson A.P.,
Korting H.C.,
Stolz W.,
StruckSauer E.,
BraunFalco O.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04119.x
Subject(s) - periplasmic space , haemophilus ducreyi , cytoplasm , bacterial outer membrane , siderophore , ultrastructure , microbiology and biotechnology , chemistry , biochemistry , bacteria , gel electrophoresis , biology , pasteurellaceae , escherichia coli , haemophilus influenzae , botany , gene , antibiotics , genetics
Protein profiles of whole cells of Haemophilus ducreyi grown in the presence or absence of the iron chelator desferal, were compared by polycrylamide gel electrophoresis. Each of four strains produced novel proteins in the range 43–160 kDa when cultured under conditions of reduced iron availability. At some sub‐inhibitory concentrations, desferal produced enhanced growth, possibly due to it functioning as an exogenous siderophore. Organisms grown under conditions of reduced iron availability ultrastructurally showed also large periplasmic spaces between cytoplasm and outer membrane.