Open AccessRapid purification of the peptide toxins microcystin‐LR and nodularin
Author(s) -
Martin Cornel,
Sivonen Kaarina,
Matern Ulrich,
Dierstein Roland,
Weckesser Jürgen
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04112.x
Subject(s) - toxin , microcystin , chromatography , elution , microcystis aeruginosa , chemistry , peptide , ammonium , microcystis , microcystin lr , biology , biochemistry , pentapeptide repeat , cyanobacteria , extraction (chemistry) , microbiology and biotechnology , bacteria , organic chemistry , genetics
An isoation procedure was developed to provide within one day microcystin‐LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammonium bicarbonate. An at least 95% purity was revealed on HPLC separation by monitoring at 214 nm. Application of the procedure to the cyclic pentapeptide toxin nodulation from Nodularia spumigena AV2 was examined.