Open Access16S rRNA sequences reveal uncultured inhabitants of a well‐studied thermal community
Author(s) -
Ward David M.,
Weller Roland,
Bateson Mary M.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04088.x
Subject(s) - 16s ribosomal rna , biology , hot spring , ribosomal rna , octopus (software) , microbial population biology , ecology , genetics , gene , bacteria , paleontology , chemistry , computational chemistry
Molecular methods are beginning to reveal inhabitants of natural microbial communities which have nerver before been cultured. Our approach involves selective cloning of naturally occurring 16S rRNA sequences as cDNA, and comparison of these sequences to a database which includes 16S rRNA sequences of isolated community members. We provide here an overview of the method and its potential for community analysis. A 16S rRNA sequence retrieved from the well‐studied hot spring cyanobacterial mat in Octopus Spring (Yellowstone National Park) is shown as an example of one contributed by an uncultured member of the community.