Open AccessIdentification by immunoblot analysis of major antigenic determinants of the anaerobic beer spoilage bacterium genus Pectinatus
Author(s) -
Hakalehto Elias,
Finne Jukka
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04038.x
Subject(s) - biology , bacteria , antigen , microbiology and biotechnology , antiserum , food spoilage , anaerobic bacteria , genetics
Antigenic determinants of the anaerobic beer spoilage bacterium Pectinatus were characterized by immunoblot analysis using surface components extracted with mild hydrochloric acid and antisera prepared against whole bacteria. The main antigenic reactivity in group I strains was associated with a major component of an apparent size between 55 and 78 kDa, whereas in group II strains a major antigen of 22 to 25 kDa was present. Based on variable reactivity of the major component two distinct subgroups (IA and IB) were discerned within group I, whereas other strains of group I were represented by single reactivities. The presence of a main protein component of variable size and antigenic properties could provide a means of detection, subgrouping and identification of Pectinatus strains in breweries, and of studying their unique surface morphology.