Open AccessAdhesion of cellulose to cell walls to Trichoderma reesei
Author(s) -
Sprey Benno
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04037.x
Subject(s) - trichoderma reesei , cellulose , cellulase , cell wall , crystallite , chemistry , materials science , biochemistry , crystallography
The attachment of Trichoderma reesei QM 9414 cell wall fractions to cellulose crystallites from cotton fibres was simulated under conditions of isopycnic density gradient centrifugation. Cell wall fractions were obtained from mechanically sheared mycelia of cellobiose‐grown Trichoderma cultures. Diameters of broken cell wall portions were 1–4 μm. Cellulose crystallites (length: 400–1 000 nm, width: 60–100 nm) were prepared from dewaxed cotton fibres using a cellulase‐complex with free‐fibre forming characteristics. The hydrolase complex was isolated from culture fluid of cellulose‐grown Trichoderma reesei . The hydrolase has endo‐ and exoglucanase activities. The structural properties of the cellulose crystallites observed by TEM are given. Mixing cellulose crystallites ( d = 1.135 g/ml) with cell wall fractions ( d = 1.080 g/ml) resulted in a hybrid band with an intermediate density of 1.105 g/ml after density gradient centrifugation. This indicates a strong binding between both components. The mode of binding cellulose to cell walls is discussed.