Open AccessCharacterization of glucoannylaserom Asperigillus terreus 4
Author(s) -
Close Anuradha,
Chatterjee B.S.,
Das Arati
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04024.x
Subject(s) - aspergillus terreus , amylopectin , amylose , enzyme , amylase , starch , chemistry , enzyme assay , alpha amylase , strain (injury) , extracellular , food science , biochemistry , biology , anatomy
A strain of Aspergillus terreus 4 was found to show extracellular amylolytic activity and the amylase was identified as glucoamylase enzyme. The optimum temperature for the enzyme activity was 60% and it was stable at this temperature for 1 h. The enzyme was optimally active at pH 5.0 and stable between pH 3.0–8.0. K m values of glucoamylase for soluble starch, amylose and amylopectin were 5.9 mg/ml, 4.8 mg/ml and 2.6 mg/ml respectively.