Open AccessLysogenic phages of Closrtidium perfringens : mapping of the chromosomal attachment sites
Author(s) -
Conard Bruno,
Cole Stewart T.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04020.x
Subject(s) - lysogenic cycle , virulence , clostridium perfringens , biology , bacteriophage , pulsed field gel electrophoresis , genetics , genome , strain (injury) , pathogenicity island , microbiology and biotechnology , circular bacterial chromosome , bacteria , gene , escherichia coli , genotype , anatomy
The sites of insertion for two lysogenic bacteriophages have been mapped on the chromosome of Clostridium perfringes strain CPN50 using two techiniques based on pulsed field gel electrophoresis. Phage Φ29 was mapped to the 1 Mb region of the 3.6 Mb genome, near nanH wich encodes a potential virulence factor, while Φ59 was found to have inserted at 2.9 Mb.