Open AccessEvaluation of a selective enrichment technique for the isolation of Campylobacter pylori
Author(s) -
Morgan Donna R.,
Mathewson J.J.,
Freedman Ray,
Kraft William G.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04016.x
Subject(s) - campylobacter , microbiology and biotechnology , agar , isolation (microbiology) , enterococcus , enrichment culture , candida albicans , campylobacter fetus , agar plate , biology , chemistry , bacteria , chromatography , antibiotics , genetics
To cultivate Campylobacter pylori from contaminated biopsy specimens, Brucella broth was supplemented with 10% fetal calf serum, 1% Vitox, 1000 units/ml polymyxin B sulfate, 10 μg/ml vancomycin, and 2 μg/ml amphotericin B. Pseudomonas aeruginosa, Candida albicans , and Enterococcus fecalis were cocultivated with C. pylori . All four strains of C. pylori were recoverable at 24 h. When 21 C. pylori strains were studied in pure culture, 86% grew in the selective enrichment medium. In a clinical study, the selective enrichment technique resulted in isolation of C. pylori from 50% of patient samples, compared with isolation from only 36% of samples with agar cultivation. The selectiove enrichment technique may be more sensitive than techniques currently employed to isolate C. pylori from gastric tissue.