Open AccessCloning of the cellulase gene from Penicillium funiculosum and its expression in Escherichia coli
Author(s) -
Sahasrabudhe N.A.,
Ranjekar P.K.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04013.x
Subject(s) - escherichia coli , cellulase , cloning (programming) , gene , biology , carboxymethyl cellulose , expression vector , chemistry , hydrolysis , gene product , microbiology and biotechnology , biochemistry , gene expression , recombinant dna , organic chemistry , computer science , programming language , sodium
A gene of Penicillium funiculosum encoding an endoglucanase was cloned and expressed in Escherichia coli using the lacZ promoter of vector pUC 18. The gene product hydrolyzed carboxymethyl cellulose and showed strong cross reactivity with P. funiculosum anticellulases.