Open AccessSsh AI restriction endonuclease from Salmonella shikmonah
Author(s) -
Miyahara Michiko,
Nakajima Katsuhisa,
Kawanishi Tsutomu,
Mise Katsutohi
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04005.x
Subject(s) - restriction enzyme , salmonella , biology , bacillus subtilis , genetics , endonuclease , recognition sequence , microbiology and biotechnology , dna , bacteria
A new type II restriction endonuclease, Ssh AI, was purified from Salmonella shikmonah TK139 of kangaroo origin. The recognition and cleavage specificity of Ssh AI was determined to be 5′‐CC/TNAGG‐3′, identical to that of Sau I from Streptomyces aureofaciens and Bsu 361 from Bacillus subtilis . Based on closely related and in part overlapping recognition specificities of Ssh AI and other restriction endonucleases, a close evolutionary relationship is proposed for all known Salmonella restriction endonucleases.