Open AccessTransformation of Staphylococcus epidermidis and other staphylococcal species with plasmid DNA by electroporation
Author(s) -
Augustin Johannes,
Götz Friedrich
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03997.x
Subject(s) - electroporation , plasmid , transformation (genetics) , staphylococcus epidermidis , dna , transformation efficiency , microbiology and biotechnology , biology , staphylococcus aureus , bacteria , chemistry , genetics , gene , agrobacterium
In this paper, the influence of various parameters on plasmid tranformation by electroporation of Staphylococcus epidermidis Tü3298 was investigated. Cell growth conditions, various concentrations and forms of plasmid DNA, field strength, pulse duration and media for electroporation and regeneration were tested. In order to obtain optimal transformation efficiency, the cells were incubated for 30 min with DNA before pulsing. With the optimized procedure, other staphylococcal species such as S. aureus, S. staphylolyticus and S. carnosus were transformed with an efficiency up to 3× 10 5 transformants per μg pC194 plasmid DNA.