Open AccessIdentification of proteins encoded in Escherichia coli hydA, hydB and analysis of the hydA locus
Author(s) -
Yamamoto Takehiro,
Tomiyama Masamitsu,
Mita Hajime,
Sode Koji,
Karube Isao
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03994.x
Subject(s) - escherichia coli , identification (biology) , biology , locus (genetics) , genetics , gene , ecology
The hydB gene of Escherichia coli , which is related with the expression of hydrogenase activity, was cloned into the plasmid (pES1). Using the maxicell protein‐labeling method, the molecular weight of hydB gene product was estimated. Comparing between the gene products from the mutant strains and that of the hydB genes cloned strains, the molecular weight of the gene product was 35 000 M r . Similarly, the molecular weight of the gene product of hydA , which had been previously cloned, was determined by maxicell analysis. The molecular weight of hydA gene product was estimated to be 80 000 M r . Using deletion analysis and Tn 1000 insertional inactivation of hydA 's function, the hydA coding region was estimated between 2.2 kb and 2.8 kb in a 3.1 kb Eco RI‐ Mlu I fragment on the recombinant plasmid pEH3.