Open AccessLocalization of an origin of replication in Corynebacterium diphtheriae broad host range plasmid pNG2 that also functions in Escherichia coli
Author(s) -
SerwoldDavis Theresa M.,
Groman Neal B.,
Chi Kao Chia
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03982.x
Subject(s) - subcloning , plasmid , corynebacterium diphtheriae , escherichia coli , biology , transformation (genetics) , shuttle vector , origin of replication , microbiology and biotechnology , cloning (programming) , multiple cloning site , genetics , dna , vector (molecular biology) , gene , virology , recombinant dna , vaccination , computer science , programming language , diphtheria
Subcloning and protoplast transformation studies identified a 2.6 kb fragment of Corynebacterium diphtheriae plasmid pNG2 which contains an origin of replication ( ori R). Molecular combination of the 2.6 kb ori R cartridge with Escherichia coli plasmid pUC18CmR enabled the E. coli cloning vector to replicate within several species of Corynebacterium host cells. A 2.6 kb plasmid formed from the ori R cartridge alone is capable of replicating in E. coli . This suggests that a single origin could be used in vectors shuttling between Corynebacterium spp. and E. coli .