Open AccessCloning of the genes encoding urease from Proteus vulgaris and sequencing of the structural genes
Author(s) -
Mörsdorf Gerhard,
Kaltwasser Heinrich
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03974.x
Subject(s) - proteus vulgaris , homology (biology) , gene , biology , open reading frame , molecular cloning , canavalia ensiformis , biochemistry , microbiology and biotechnology , nucleic acid sequence , peptide sequence , cloning (programming) , cysteine , urease , proteus , enzyme , genetics , escherichia coli , computer science , programming language
A fragment of chromosomal DNA from Proteus vulgaris encoding urease was cloned and expressed in Escherchia coli . A 3 kbp region was sequenced and revealed three open reading frames with homology to jack ( Canavalia ensiformis ) urease. The smallest protein (11 kDa) was homologous to the N‐terminus of the plant enzyme and the largest polypeptide (61 kDa) corresponded to the C‐terminus. The large protein contained conserved regions and a cysteine residue which is known to be catalytically important in the plant enzyme. A protein of 12 kDa showed homology to residues 132 to 237 of jack bean urease.