Open AccessSimplified procedure for purification of Bordetella bronchiseptica dermonecrotic toxin
Author(s) -
Horiguchi Yasuhiko,
Nakai Toyotsugu,
Kume Katsumi
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03969.x
Subject(s) - bordetella bronchiseptica , chromatography , toxin , bordetella , sodium dodecyl sulfate , gel electrophoresis , polyacrylamide gel electrophoresis , column chromatography , chemistry , high performance liquid chromatography , microbiology and biotechnology , biology , biochemistry , bacteria , bordetella pertussis , genetics , enzyme
Bordetella bronchiseptica dermonecrotic toxin was purified by a simplified method. The method consisted of SP Toyopearl 650M chromatography and high performance liquid chromatography on a TSK gel G3000SW column. 47.5% of the activity of the crude cell extract was recovered. The purified toxin behaved as a homogeneous protein in sodium dodecyl sulfate polyacrylamide gel electrophoresis, high performance liquid chromatography, and agar gel double diffusion tests.