Open AccessCloning and expression of chromosomally and plasmid‐encoded glyceraldehyde‐3‐phosphate dehydrogenase genes from the chemoautotroph Alcaligenes eutrophys
Author(s) -
Windhövel Ute,
Bowien Botho
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03967.x
Subject(s) - biology , plasmid , gene , glyceraldehyde 3 phosphate dehydrogenase , gene cluster , heterologous expression , cloning (programming) , microbiology and biotechnology , escherichia coli , genetics , molecular cloning , dehydrogenase , gene expression , biochemistry , recombinant dna , enzyme , computer science , programming language
Hybridizations using heterologous glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) gene probes suggested the existence of three GAPDH genes in Alcaligenes eutrophus H16. Two of these, located on the chromosome and the megaplasmid pHG1 of the organism, respectively, mapped about 2.5 kilobase pairs (kb) downstream of the two duplicated CO 2 fixation gene clusters ( cfx genes). They were identified as GAPDH genes ( cfxG c and cfxG p ) by cloning and expression in Escherichia coli . These genes encode GAPDH isoenzymes functioning in the Calvin cycle. The third gene ( gap ) is chromosomally encoded but not linked to the cfx c cluster. Its product is probably involved in heterotrophic carbon metabolism.