Open AccessDerivatives of Rhodobacter capsulatus strain AD2 cured of their endogenous plasmid are unable to utilize nitrate
Author(s) -
Willison John C.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03966.x
Subject(s) - rhodobacter , plasmid , nitrate reductase , nitrate , strain (injury) , endogeny , biology , microbiology and biotechnology , chemistry , biochemistry , gene , mutant , ecology , anatomy
Derivatives of Rhodobacter capsulatus AD2 unable to grow with nitrate as sole N source were isolated after conjugation with a plasmid containing a cloned 3.4 kb Hin dIII fragment from the endogenous plasmid of this strain. These derivatives lacked the M r 74 × 10 6 plasmid found in the wild‐type, and failed to revert to growth on nitrate. Cultures of the plasmid‐cured strains also lacked dissimilatory nitrate reductase activity, suggesting that genes required for both assimilatory and dissimilatory nitrate reduction are located on the endogenous plasmid.