Comparison of forward and backwards methods of cell cycle analysis

We tend to look at hfe as a progression from youth to old age. This is reflected in our study of the bacterial diwsion cycle, where newborn cells have an age of 0.0 and progress through the division cycle, finally dividing at age 1.0. There has been much effort expended in deterrmnlng the rate and pattern of innsynthesis during the d m sion cycle, and synchronization of cells has been the most common method for cell cycle analysis [1]. Synchromzation may be called a forward method, as it directly reflects the increase ra cell age in the usual manner; a synchromzed population is one in winch all cells are the same age during the diviston cycle. The rates of synthesis of various molecules during the divtslon cycle may be obtained by talang samples from cells of all ages from newborn to dividing cells. Although there has been a great deal of work on synchromzatmn, 1 am unaware of any generally accepted result analyzing the division cycle that was discovered