Open AccessIdentification of the gene encoding the regulatory protein B of soluble methane monooxygenase
Author(s) -
Pilkington S.J.,
Salmond G.P.C.,
Murrell J.C.,
Dalton H.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03914.x
Subject(s) - methane monooxygenase , gene , gene cluster , regulator gene , monooxygenase , biology , biochemistry , chemistry , genetics , microbiology and biotechnology , regulation of gene expression , enzyme , cytochrome p450
Purification of the regulatory protein B of the soluble methane monooxygenase complex from Methylococcus capsulatus (Bath) has revealed that the organism contains two forms of this protein, one of which appears to be a carboxy‐terminal truncate. Protein sequencing has confirmed the identity of these two proteins and allowed the identification of the gene encoding protein B on the methane monooxygenase gene cluster.