Open AccessCarboxyl‐terminal deletion analysis of the Streptococcus mutans glucosyltransferase‐I enzyme
Author(s) -
Kato Chiaki,
Kuramitsu Howard K.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03906.x
Subject(s) - glucosyltransferase , streptococcus mutans , sucrase , enzyme , glucosyltransferases , biochemistry , chemistry , microbiology and biotechnology , streptococcaceae , bacteria , biology , genetics , antibiotics
Sequential deletion of the carboxyl‐terminal amino acids (including the six direct repeating units) of the glucosyltransferase‐I (GTF‐I) enzyme of Streptococcus mutans revealed differential effects on sucrase and GTF activities. Removal of all but one repeating unit resulted in a truncated enzyme with significant sucrase activity but no detectable GTF activity. These results are compatible with the presence of two functional domains in the enzyme.