Open AccessA Clostridium cellulovorans gene, engD , codes for both endo‐β‐1,4‐glucanase and cellobiosidase activities
Author(s) -
Hamamoto Tetsuo,
Shoseyov Oded,
Foong Frances,
Doi Roy H.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03903.x
Subject(s) - glucanase , plasmid , microbiology and biotechnology , molecular cloning , escherichia coli , biology , gene , clone (java method) , enzyme , biochemistry , chemistry , peptide sequence
A 5.8 kbp DNA fragment from Clostridium cellulovorans (ATCC 35296) containing endo‐β‐1,4‐glucanase (1,4‐β‐ d ‐glucan glucanohydrolase, carboxymethylcellulase, CMCase; EC 3.2.1.4) gene, engD was cloned in Escherichia coli . The clone harboring a subcloned 3.8 kb fragment in plasmid, pEQ52V, produced an enzyme that showed both endo‐β‐1,4‐glucanase activity as well as cellobiosidase activity. Zymograms with the engD encoded enzyme with carboxymethyl‐cellulose as the substrate indicated that the molecular mass of the active protein was 50 000.