Open AccessCloning and expression of the plasmid‐encoded benzene dioxygenase genes from Pseudomonas putida ML2
Author(s) -
Meng Hai,
Mason Jeremy R.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03899.x
Subject(s) - pseudomonas putida , dioxygenase , plasmid , escherichia coli , gene , heterologous expression , pseudomonas , biology , molecular cloning , cloning (programming) , microbiology and biotechnology , heterologous , recombinant dna , bacteria , gene expression , biochemistry , genetics , computer science , programming language
Hybridization using heterologous dioxygenase gene probes indicated the presence of the genes encoding the enzyme benzene dioxygenase on a 112 kb plasmid from Pseudomonas putida ML2. They were identified as benzene dioxygenase genes ( bed ABC1C2) by cloning in Escherichia coli and analysis of expression by Western blotting using antibodies raised to the four polypeptides of purified benzene dioxygenase.