Open AccessElectrotransfection of Escherichia coli with ?gt10 DNA
Author(s) -
Powell Richard,
Heery David M.,
Gan Frank,
Kieran Dunican L.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03893.x
Subject(s) - electroporation , escherichia coli , dna , microbiology and biotechnology , in vitro , transfection , biology , cloning (programming) , chemistry , biochemistry , gene , computer science , programming language
We have used electroporation to introduce ?gt10 DNA into E. coli C600 (Electrotransfection). We obtained approximately 10 7 pfu (plaque forming units) per μg of ?gt10 DNA. This frequency is 100‐fold higher than the maximum reported for classical calcium chloride‐induced transfection. We have also compared electrotransfection with in vitro packaging in cloning experiments using relatively small amounts (? 10 ng) of DNA. Our results slow that electrotransfection can generate approximately 1000‐fold more plaques than in vitro packaging at these concentrations.