Open AccessPlasmid transformation of Bacillus cereus on cellophane membranes
Author(s) -
Sabelnikov A.G.,
Ulyashov L.V.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03874.x
Subject(s) - bacillus cereus , transformation (genetics) , plasmid , cellophane , nutrient agar , bacteria , biology , microbiology and biotechnology , cereus , membrane , dna , agar , gene , chemistry , genetics , organic chemistry
A simple approach to test the ability of bacteria to undergo natural genetic transformation is suggested. The basic feature of the approach is the cultivation of bacterial cells in the presence of exogenous (plasmid) DNA on cellphone membranes placed successively on nutrient and selective agar. Using this approach the ability of Bacillus cereus for “natural” genetic transformation was detected. Transformation frequencies varied from 10 −8 to 10 −6 .