Open AccessMonoclonal antibodies against serotype specific and conserved epitopes in morphotype E Escherichia coli flagellins
Author(s) -
Schoenhals Gary,
Whitfield Chris
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03873.x
Subject(s) - epitope , monoclonal antibody , serotype , biology , virology , epitope mapping , escherichia coli , antibody , monoclonal , microbiology and biotechnology , genetics , gene
Monoclonal antibodies (mAbs) were used to examine the interrelationships between morphologically identical flagellar filaments from Escherichia coli H serotype belonging to morphotype E. Serotype specific mAbs recognised epitopes exposed on the surface of flagellar filaments from H1, H7, H23, H49 and H51, but were inaccessible to immunolabelling in H45. Several mAbs which recognised conserved epitopes were also examined. mAb 7–56.1 recognised an epitope present in all morphotype E flagellins but not expressed on the filaments surface. Similarly, mAb 1–5.1 recognised an internal epitope shared only by serotypes H1 and H2. Serotype H23 expressed a surface epitope which was present but not surface exposed in H7, H1 and H45 filaments.