Open AccessRemarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii
Author(s) -
Asai Takashi,
Suzuki Yasuhiro
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03867.x
Subject(s) - virulence , toxoplasma gondii , nucleoside triphosphate , hydrolase , nucleoside , strain (injury) , atpase , nucleotide , biology , dithiothreitol , enzyme , microbiology and biotechnology , pyrophosphatases , atp hydrolysis , adenosine triphosphate , biochemistry , chemistry , gene , genetics , anatomy , antibody
Dithiothreitol‐dependent MgATP 2 and MgADP − hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically un tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of V max and K m obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.