Open AccessEffect of prior treatment with Clostridium perfringens epsilon toxin inactivated by various agents on lethal, pressor and contractile activities of the toxin
Author(s) -
Nagahama Masahiro,
Takahashi Toshihiro,
Sakurai Jun
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03862.x
Subject(s) - toxin , succinic anhydride , clostridium perfringens , chemistry , carbodiimide , glycine , tetranitromethane , biochemistry , amino acid , microbiology and biotechnology , pharmacology , bacteria , biology , organic chemistry , enzyme , genetics
Lethal and pressor activities, and the contractile responses of rai isolated ileum to Clostridium perfrigens epsilon toxin, were significantly prevented by the prior administration of epsilon toxin inactivated by 1‐ethyl‐3(3‐diethyly‐aminopropyl) carbodiimide in the presence of glycine methyl ester (EDC), 2,4,6‐trinitrobenzene sulfonic acid (TNBS), succinic anhydride (SA) and ethoxyformic anhydride (EFA). Howerver, the prior administration of the toxin inactivated by N ‐acetylimidazole (NaI), tetranitromethane(TNM) and N ‐bromosuccinimide (NBS) resulted in no inhibition of these biological activities. These data suggest that the toxin interacts with specific site(s) on target organs or tissues. The relationship between amino acid residues and the actions of the toxin is described.