Open AccessPromoters of major Escherichia coli heat shock genes seem non‐functional in Bacillus subtilis
Author(s) -
Wetzstein Michael,
Schumann Wolfgang
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03861.x
Subject(s) - bacillus subtilis , promoter , escherichia coli , gene , heat shock , heat shock protein , shock (circulatory) , biology , sigma factor , microbiology and biotechnology , chemistry , genetics , bacteria , gene expression , medicine
To find out whether Escherichia coli heat shock promoters are recognized in Bacillus subtilis , the regulatory regions including the heat shock promoters of the main heat shock genes dnaKJ, lon, groES , and htpG were fused to the indicator genes lacZ and cat . Whereas all transcriptional fusions were expressed in E. coli at low temperature and transient increases of β‐galactosidase activity could be measured at the inducting temperature, no enzymatic activity was found in B. subtilis . This indicates that E. coli heat shock promoters are nonfunctional in B. subtilis .