Open AccessCloning of Klebsiella pneumoniae pqq genes and PQQ biosynthesis in Escherichia coli
Author(s) -
Meulenberg J.J.M.,
Sellink E.,
Loenen W.A.M.,
Riegman N.H.,
Kleef M.,
Postma P.W.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03847.x
Subject(s) - klebsiella pneumoniae , escherichia coli , pyrroloquinoline quinone , cloning (programming) , gene , mutant , biology , clone (java method) , microbiology and biotechnology , molecular cloning , biosynthesis , enterobacteriaceae , genetics , biochemistry , peptide sequence , cofactor , enzyme , computer science , programming language
We have cloned genes from Klebsiella pneumoniae which are required for pyrroloquinoline quinone (PQQ) biosynthesis. The cloned 6.7 kb fragment can complement several chromosomal pqq mutants. Escherichia coli strains are unable to synthesize PQQ but E. coli strains containing the cloned 6.7 kb K. pneumoniae fragment can synthesize PQQ in large amounts and E. coli pts mutants can be complemented on minimal glucose medium by this clone.