Open AccessStrategies for improved lignin peroxidase production in agitated pellet cultures of Phanerochaete chrysosporium and the use of a novel inducer
Author(s) -
Liebeskind M.,
Höcker H.,
Wandrey C.,
Jäger A.G.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03845.x
Subject(s) - phanerochaete , lignin , peroxidase , lignin peroxidase , inducer , incubation , chrysosporium , chemistry , glycerol , biochemistry , extracellular , food science , carbon source , enzyme , organic chemistry , gene
The production of extracellular H 2 O 2 ‐dependent lignin peroxidase by the basidiomycete Phanerochaete chrysosporium in agitated submerged cultures was improved by the addition of a novel inducer and by a particular agitation and temperature program. The combination of an exact shift of agitation rate from higher to lower speed after the onset of secondary metabolism, the simultaneous decrease of the incubation temperature from 39 to 25°C and the supply of appropriate concentrations of dimethoxybenzylamine instead of veratryl alcohol resulted in a 5‐fold increase in enzyme production compared to control experiments. With this method, lignin peroxidase activities at a maximum of 1312 U 1 −1 were obtained usign glycerol as sole carbon source and nitrogen‐limited media.