Open AccessIntergeneric protplast fusion between xylanase producing Bacillus subtilis LYT and Corynebacterium acetoacidphilum ATCC 21476
Author(s) -
Deb J.K.,
Malik Sundeep,
Ghosh V.K.,
Mathai S.,
Sethi Renu
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03838.x
Subject(s) - bacillus subtilis , protoplast , xylanase , biology , microbiology and biotechnology , lysine , bacteria , corynebacterium , hybrid , bacillus (shape) , biochemistry , genetics , botany , enzyme , amino acid
Hybrids between a strain of Bacillus subtilis isolated in our laboratory and having the ability to degrade xylan and other complex polysaccharides and Corynebacterium acetoacidophilum , a lysine producer, were prepared by protoplast fusion. Based on distinctive parental biochemical characteristics the fusants were grouped into 9 categories, viz. BC1 through BC9. Three of the hybrids, BC5, BC7a and BC7b, were tested for their ability to produce xylanase and lysine. Both BC7a and BC7b produced xylanase but BC5 did not, however all of them produced lysine albeit to different degrees. These results demonstrate that intergeneric gene transfer takes place through protoplast fusion between these 2 important genera of bacteria and some of the fusants inherit the useful traits of both the parents.