Open AccessA cloned chromosomal DNA fragment which differentiates Yersinia pestis from Yersinia pseudotuberculosis and Yersinia enterocolitica
Author(s) -
Bardarov S.S.,
Sirakova T.D.,
Kriakov J.,
Karakashyan A.,
Markov K.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03836.x
Subject(s) - genetics , biology , fragment (logic) , yersinia enterocolitica , dna , microbiology and biotechnology , bacteria , computer science , programming language
Chromosomal DNA from reference Yersinia strains was digested individually with 9 restriction endonucleases. DNA fragments were separated and analyzed by electrophoresis through agarose gels. The clearest fragment patterns were obtained when Eco RI was employed. the Y. pestis fragment pattern obtained after the use of this enzyme showed the presence of a unique DNA fragment with molecular mass 1400 bp. This DNA fragment was cloned, purified, labeled with 32 P and then used to probe Eco RI digests of all three Yersinia species. A strong hybridization signal was obtained with Y. pestis strain. No such signal was found with Y. pseudotuberculosis or Y. enterocolitica . These results indicate that the DNA fragment is species specific and could be used as a diagnostic DNA probe for Y. pestis .