Open AccessSolubilisation of a cell wall bound invertase in Aspergillus nidulans
Author(s) -
Vainstein Marilene Henning,
Peberdy John F.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03834.x
Subject(s) - invertase , aspergillus nidulans , mycelium , sucrose , dithiothreitol , biochemistry , chemistry , extracellular , cell wall , enzyme , aspergillus , hydrolysis , biology , microbiology and biotechnology , botany , mutant , gene
Aspergillus nidulans produces an extracellular invertase when incubated in the presence of sucrose and about half of the activity produced was found to be associated with the mycelium. Sixty percent of this mycelial invertase could be solubilised by simple mechanical disruption. Among the agents tested for solubilisation of invertase, proteinase K and dithiothreitol were the most effective.