Open AccessHigh transformation frequency of nonsporulating mutants of Streptomyces griseus
Author(s) -
Daza Antonio,
Martin Juan Francisco,
Gil JoséAntonio
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03833.x
Subject(s) - streptomyces griseus , mutant , transformation (genetics) , plasmid , biology , mutagenesis , microbiology and biotechnology , transformation efficiency , streptomycetaceae , dna , bacteria , streptomyces , genetics , gene , actinomycetales , agrobacterium
Three different bld mutants from S. griseus ATCC 10137 were isolated by nitrosoguanidine mutagenesis. They simultaneously lost the capability of antibiotic production and the formation of pigments. The three bld mutants were differently affected by different carbon sourcs. Two of these mutants showed a high efficiency of transformation with several plasmid vectors, in contrast to the low efficiency of transformation showed by the wild type. We showed that S. griseus ATCC 10137 and the three bld mutants possess and enzymatic activity that protects their DNAs against the digestion by Sac I. Antibiotic and pigment production, and low transformability with plasmid DNA were together restored in spontaneous spo + revertants.