Open AccessDegradation of 3‐chlorobiphenyl by in vivo constructed hybrid pseudomonads
Author(s) -
Mokross Heinrich,
Schmidt Eberhard,
Reineke Walter
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03819.x
Subject(s) - degradation (telecommunications) , in vivo , chemistry , biology , computer science , microbiology and biotechnology , telecommunications
3‐Chlorobiphenyl‐degrading bacteria were obtained from the mating between Pseudomonas putida strain BN10 and Pseudomonas sp. strain B13. Strains such as BN210 resulted from the transfer of the genes coding the enzyme sequence for the degradation of chlorocatechols from B13 into BN10, whereas B13 derivatives such as B131 have acquired the biphenyl degradation sequence from BN10. During growth of the hybrid strains on 3‐chlorobiphenyl 90% chloride was released. Activities of phenylcatechol 2,3‐dioxygenase, benzoate dioxygenase, catechol 1,2‐dioxygenase, chloromuconate cyloisomerase and 4‐carboxymethyl‐enebut‐2‐en‐4‐olide hydrolase were found in 3‐chlorobiphenyl‐grown cells. The hybrid strains were found to convert some congeners of the Aroclor 1221 mixture such as mono‐ and dichloro‐substituted biphenyls.