Open AccessAerobic respiration in sulfate‐reducing bacteria *
Author(s) -
Dilling Waltraud,
Cypionka Heribet
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03809.x
Subject(s) - thiosulfate , sulfite , chemistry , respiration , cellular respiration , cyanide , anaerobic respiration , formate , sulfur , biochemistry , sulfide , sulfate , desulfovibrio , oxygen , inorganic chemistry , bacteria , biology , organic chemistry , catalysis , mitochondrion , botany , genetics
Cultures of Disulfovibrio desulfuricans strain CSN (incubated in a sulfide‐ and sulfate‐free medium) reduced up to 5 mM O 2 with H 2 as electron donor. Aerobic respiration was not coupled with growth, but resulted in ATP formation. Washed cells incubated in H 2 ‐saturated phosphate buffer revealed respiration rates of up to 250 nmol O 2 min −1 mg protein −1 . The uncoupler carbonyl‐cyanide m ‐chlorophenylhydrazone (CCCP) stimulated the respiration rate and abolished ATP formation. The terminal oxidase has not yet been identified. Respiration was microaerophilic, insensitive to cyanide and azide, but inhibited after heat treatment of the cells (80°C for 10 min). The ph optimum was at pH 6 with less than 50% activity at pH 4.5 and pH 9. Besides H 2 , organic eletron donors (formate, ethanol, lactate or pyruvate) and inorganic sulfur compounds (H 2 S, thiosulfate, sulfite) were used as electron donors for aerobic respiration. Sulfite and thiosulfate were oxidized completely to sulfate. The capability of aerobic respiration was also detected in Desulfovibrio vulgaris, D. sulfidismutans, Desulfobacterium autotrophim, Desulfobulbus propionicus , and Desulfococcus multivorans .