Open AccessIntegration of an unstable plasmid into the chromosome of Lactobacillus plantarum
Author(s) -
Rixon Jane E.,
Hazlewood Geoffrey P.,
Gilbert Harry J.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03806.x
Subject(s) - plasmid , lactobacillus plantarum , biology , chromosome , bacteria , microbiology and biotechnology , population , genetics , genomic dna , lactobacillus , dna , lactic acid , gene , demography , sociology
A 2.3 kb Eco RI restriction fragment of Lactobacillus plantarum genomic DNA was cloned into pSA3 to generate pJRI. Both pSA3 and pJR1 were transformed into L. plantarum . Growth of the transformants in the absence of the selection pressure, erythromycin (both plasmids confer Em r ), led to a stable sub‐population of EM r bacteria in which pSA3 and pJR1 had integrated into the L. plantarum genome. Amplification of the chromosomally located plasmids was observed when the organism was grown in the presence of erythromycin. The integrated plasmid sequences were stably maintained in the absence of selection pressure, and did not affect the growth rate of the lactic acid bacterium in rich media.