Open AccessSizing of the Leptospira genome by pulsed‐field agarose gel electrophoresis
Author(s) -
Baril Céline,
Girons Isabelle Saint
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03804.x
Subject(s) - leptospira , pulsed field gel electrophoresis , agarose gel electrophoresis , sizing , gel electrophoresis , agarose , genome , chromatography , chemistry , biology , computational biology , microbiology and biotechnology , leptospirosis , genetics , virology , dna , gene , genotype , organic chemistry
Pulsed‐field gel electrophoresis allowed the determination of the size of the genome of Leptospira , a bacterium of the spirochete family. The three restriction enzymes, Not I (5′ GC/GGCCGC), Nhe I (5′‐G/CTAGC), Apa I (5′‐GGGCC/C) generated DNA fragments of suitable size. The results are compatible with a size of 5000 kb for the chromosome of both the pathogenic and the saprophytic species of Leptospira .