Open AccessHydrogen production during fermentation of acetoin and acetylene by Pelobacter acetylenicus
Author(s) -
Seitz H.J.,
Siñeriz F.,
Schink B.,
Conrad R.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03802.x
Subject(s) - acetoin , formate , chemistry , acetate kinase , acetylene , fermentation , ethanol , substrate (aquarium) , organic chemistry , biochemistry , nuclear chemistry , catalysis , biology , ecology , escherichia coli , gene
Pelobacter acetylenicus accumulated only small amounts of H 2 (< 3.5 kPa) during fermentation of acetoin or acetylene to acetate and ethanol. Formate was also produced in small amounts (< 0.5 mM). Growth on acetoin was retarded by addition of ethanol, but not by addition of H 2 or formate. However, addition of H 2 and/or formate resulted in increased production of 2,3‐butanediol, whereas addition of H 2 ‐scavenging Methanospirillum hungatei resulted in production of acetate plus H 2 (as CH 4 ) instead of acetate plus ethanol. Growth yields were consistent with acetate kinase as the sole ATP‐generating reaction. The results are discussed with respect to thermodynamics and ATP synthesis during substrate conversion.