Open AccessThe nucleotide sequence of an α‐amylase gene from an alkalopsychrotrophic Micrococcus sp
Author(s) -
Kimura Takuhei,
Horikoshi Koki
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03795.x
Subject(s) - nucleic acid sequence , biology , gene , plasmid , microbiology and biotechnology , escherichia coli , open reading frame , micrococcus , recombinant dna , peptide sequence , amylase , dna , nucleotide , biochemistry , genetics , enzyme , bacteria
An α‐amylase gene from Micrococcus sp. 207 was cloned into Escherichia coli JM101 using the vector pHSG399. The constructed recombinant plasmid fragment derived from strain 207 DNA. The cloned amylase isolated from E. coli JM101 (pYK63) produced mainly maltotetraose from starch, and exhibited temperature and pH activity profiles closely similar to those of the enzyme from the original strain. Nucleotide sequence analysis of the cloned DNA fragment revealed one open reading frame containing the gene which consisted of 3312 bp (1104 amino acids). When compared with several other α‐amylases, three consensus sequences were identified in the region of the active site. About 300 amino acid residues were present both upstream and downstream of the active site region.