Open AccessThe cloning and expression of a gene encoding haemolytic activity from the fish pathogen Renibacterium salmoninarum
Author(s) -
Evenden A.J.,
Gilpin M.L.,
Munn C.B.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03794.x
Subject(s) - cosmid , biology , molecular cloning , cloning (programming) , microbiology and biotechnology , gene , southern blot , escherichia coli , pathogen , sequence analysis , gene expression , genetics , computer science , programming language
A gene encoding haemolytic activity from Renobacterium salmoninarum (strain PPD)) was cloned into Escherichia coli using the cosmid vector pHC79, and subsequently subcloned on a 1.6 kbp SA /I fragment into pBR328. Southern blot hybridisation revealed that a homologous sequence is found in other strains of R. salmoninarum .