Open AccessCharacterisation of Bacteroides from sheep periodontal disease by SDS‐PAGE of outer membrane proteins
Author(s) -
McCourtie Jane,
Poxton Ian R.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb03789.x
Subject(s) - bacteroidaceae , biology , microbiology and biotechnology , bacterial outer membrane , bacteroides , subspecies , bacteria , periodontal disease , porphyromonas gingivalis , flora (microbiology) , homogeneous , escherichia coli , biochemistry , zoology , gene , genetics , medicine , physics , thermodynamics
Isolates of Bacteroides species obtained from a longitudinal study of developing periodontal disease in sheep were analysed by SDS‐PAGE. Protein profiles of Sarkosyl‐insoluble outer membrane extracts were compared within groups of isolates which had already been defined by conventional biochemical techniques. Heterogeneity was exhibited within most groups. Isolates of B. gingivalis and B. asaccharolyticus shown to be similar to human isolates by conventional biochemical tests, gave different protein profiles from the respective type cultures. The sheep B. gingivalis ‐like isolates were however homogeneous, while the B. asaccharolyticus ‐like organisms could be divided into 3 subgroups. SDS‐PAGE appears to be a useful tool for the examination of bacterial flora and recognition of subgroups or subspecies.